Part of the Oxford Instruments Group
Welcome to the Imaris 8.2 release notes. Please have a look to theoverview of Imaris Release notesfor information about prior released features and fixed bugs.
Version Date: December 16, 2015
Imaris8.2 has a lineage tracking algorithm that allows to track dividing cells. The lineage tracking algorithm is available on the tracking tab of Spots, Surfaces, Cells or Filaments.
The screenshot below shows spots tracked with the lineage tracking algorithm and colored by “TrackID”. Spots with the same color descend from the same mother cell.
To complement the lineage tracking functionality Imaris8.2 has a new track editor that displays tracks as “lineage tree” in such a way that the number of intersections in the display is minimal (often zero).
The track editor provides useful functionally to manually edit tracks using "connect" and "disconnect" and to arrange the lineage tree in different ways by moving tracks left or right or flipping the orientation of branches. The new track editor has the functionality to overlay colors on the tracks. This can be used to display any statistical measurement value as a color on the tracks. It enables for example a display of the intensity of a second channel for each tracked object.
“对象标签”feature described in the next section can be used to highlight certain parts of a lineage tree as shown in this figure:
Users can add labels to 3D objects such as spots, surfaces, cells, vesicles, etc. The labels can be used to color code objects. They are also added to the statistics of each object as a factor that appears in the statistics tables and export. In the screenshot below the blue objects have been labeled as “Fast” and the orange objects have been labeled as “Slow”. The colors are used for rendering in the 3D view. The label names are displayed in the statistics.
Saving files in version 8.2 is significantly faster (up to a factor of 5) than previous versions due to multithreaded gzip compression of image data. This applies to both Imaris and ImarisFileConverter.
The objectIDs that Imaris assigns to spots, surfaces, cells, nuclei and vesicles do not change when an object is deleted or added or the whole container is copied. As a consequence of this the IDs no longer necessarily span an entire range of numbers. Suppose you have 4 spots with IDs 1,2,3,4 and you delete the one with ID 2, then the remaining IDs are 1,3,4.
There is a new function “GetIds” which returns the ObjectIDs used in statistics.
| Fixed 65 Bugs in Imaris 8.2.0 | ||
| 9120 | Filament 'Pt Total Number of Points' statistic is 0 on re-loading a dataset | |
| 9080 | Ids of statistics are always -1 (Mac only) | |
| 9060 | Imaris should attempt to start the DataService when opened | |
| 9056 | Trying to open a file on a remote server which no longer exists will lead to an infinite loop requesting for credentials | |
| 8990 | 'Migrate Storage Location' fails with error message "Invalid input string" | |
| 8988 | Available color options for Tracks change if you switch to Settings tab and go back to Color tab | |
| 8987 | If the file resulting from a batch run is too long for the operating system then batch will fail | |
| 8955 | Store remote image directly from Surpass does not prompt for credentials | |
| 8936 | Python not functioning in osx 10.11 | |
| 8918 | Crash when deleting cell | |
| 8893 | Crash when opening image with batch results but no license to view results | |
| 8892 | Cell Cytoplasm Number of Vesicles should be unitless | |
| 8887 | If the first file added for a remote location is not present Imaris cannot add additional files from this location | |
| 8883 | Export function does not allow to overwrite existing file | |
| 8869 | Numbering for channel indexes in Cells/Nuclei do not agree with Vesicles in creation parameters | |
| 8862 | Fileconverter 8.1.2 adds blank time frame to sequence when converting ZVI file | |
| 8859 | No result from saving Coloc Histogram | |
| 8845 | export results from batch statistics fails if group folder has a colon | |
| 8830 | Arena shortcuts to add 'Group' and 'Image' items are not working | |
| 8820 | Statistics search crashes Imaris when searching for Track ID | |
| 8817 | Search with no folder currently selected in Arena will cause a crash | |
| 8811 | Altered Keyframe Animation is not saved by 'Store' | |
| 8789 | Timebar lost upon toggling Full screen mode | |
| 8786 | Annotations cannot be placed within the image | |
| 8778 | Cells creation parameters are not displayed correctly from preferences dialog | |
| 8766 | Manual Filament Autopath Crash after Cancel | |
| 8764 | Increase the size of Imaris admin popup | |
| 8763 | Moving DataStorage with ImarisAdmin to Remote location leads to unavailable images | |
| 8760 | Path to "Python 2.7 application" does not allow spaces | |
| 8758 | Double clicking image to open in Arena view is not possible if the properties tab is not visible | |
| 8751 | Add Channel, Add Time Points and Add Slices cause a crash when multiple files are selected | |
| 8750 | Evaluation dialog shows ugly formatting and OS's that are no longer supported | |
| 8749 | Arena => Zoom drop down is to narrow and not full text is visible | |
| 8747 | Changing default values in ImarisAdmin should propogate c... | |
| 8733 | Arena's Search doesn't work if there's a space in the name | |
| 8709 | No Tracks are selected when on the Filter tab with no filters added | |
| 8706 | subsequent "Store" does not "save" delete channels. | |
| 8704 | No error message when unsupported file opened in Surpass | |
| 8698 | Shift + cmd + p shows Annotation tab in Arena view | |
| 8687 | Remove QTVR options from Animation Export Window | |
| 8681 | Delete a Cell with Nuclei from Selection filter causes Crash | |
| 8676 | If the store of Arena fails during Migration the user should be notified | |
| 8673 | Cancel button not accessible during Arena Store | |
| 8672 | Currently selected channel for popups under Image Processing selection should be clearer | |
| 8662 | Imaris crashes when deleting an object referenced in a Vantage plot | |
| 8635 | AVI RAW export (via "Save As...") is not really a "RAW" format | |
| 8580 | Settings ignored when adding Image to Arena view | |
| 8559 | LSMExcitation and LSMEmission values no longer read for some files in which Imaris could previously read this value | |
| 8489 | Statistics calculation in Wizard in endless loop or crashes if no statistics selected | |
| 8446 | For some ND2 the z calibration is not read in correctly | |
| 8434 | Tiff reader from within Arena ignores settings | |
| 8391 | Resample settings ignored in Add Time Points | |
| 8355 | After store of Vantage parameters, creation tab shows creation parameters from surpass view | |
| 8305 | Imaris will crash when user is doing manual tracking with Spot and Dragon tail is activated | |
| 8303 | Surpass Image Store accepts any remote credentials for the first image | |
| 8124 | Preferences Dialog crashes when only Vantage license is enabled | |
| 8121 | Last Timepoints not saved for Animation or TimeMovie | |
| 8049 | Default size of the preferences area should be smaller | |
| 7847 | Spot to Spot Closest Distance Xtension has not sufficient memory to work on a customer dataset | |
| 7691 | ims file with broken Filament segment cannot be opened in 7.7.0 or newer versions but can be in 7.6.5 | |
| 7654 | Some ZVI files which open correctly in Windows cannot be opened on Mac | |
| 7632 | Imaris crashes when setting spot time index to large value | |
| 7250 | ZVI Tiled time series can not be opened in Imaris | |
| 7247 | Cannot save (overwrite) IMS-file after crop time | |
| 7236 | Auto-Connect does not connect the last time point | |
| 7002 | Imaris XT "Project to 2D" fail with files larger than 2000 pixels | |
| Fixed 2 Bugs (since Imaris 8.2.0) | |
| 8783 | Timestamps from LIF files not read correctly (from new LAX software) |
| 9324 | License issues with Imaris 8.2.0 Track vs. LineageTrack |
| 9328 | When RecordingDate value for Leica files is not present Imaris uses '-4713-11-24 00:00:00.000' |
© Oxford Instruments 2022
